Coordinated regulation of Myc trans-activation targets by Polycomb and the Trithorax group protein Ash1.

ABSTRACT:

Background

The Myc oncoprotein is a transcriptional regulator whose function is essential for normal development. Myc is capable of binding to 10% of the mammalian genome, and it is unclear how a developing embryo controls the DNA binding of its abundant Myc proteins in order to avoid Myc's potential for inducing tumorigenesis.

Results

To identify chromatin binding proteins with a potential role in controlling Myc activity, we established a genetic assay for dMyc activity in Drosophila. We conducted a genome-wide screen using this assay, and identified the Trithorax Group protein Ash1 as a modifier of dMyc activity. Ash1 is a histone methyltransferase known for its role in opposing repression by Polycomb. Using RNAi in the embryo and Affymetrix microarrays, we show that ash1

SUBMITTER: Goodliffe JM 

PROVIDER: S-EPMC1887537 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature