False positive PCR detection of Tropheryma whipplei in the saliva of healthy people.

ABSTRACT:

Background

Tropheryma whipplei, the agent of Whipple's disease (WD), has been recently isolated and the genomes of two isolates have been fully sequenced. Previous diagnosis tools for the diagnosis of the disease used sequence analysis of the 16S rRNA gene. Using this target gene, the high percentage of detection of the bacterium in saliva of healthy people was in contrast to the negative results obtained with specific target genes. The aim of our study was to compare previously published primers targeting the 16S rRNA gene to real-time PCR with Taqman* probes targeting specific repeat genes only found in the genome of T. whipplei in a series of 57 saliva from healthy people.

Results

Although the specific real-time PCR assays with both primers and probes were negative for al

SUBMITTER: Rolain JM 

PROVIDER: S-EPMC1890548 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature