Discriminators of mouse bladder response to intravesical Bacillus Calmette-Guerin (BCG).

ABSTRACT:

Background

Intravesical Bacillus Calmette-Guerin (BCG) is an effective treatment for bladder superficial carcinoma and it is being tested in interstitial cystitis patients, but its precise mechanism of action remains poorly understood. It is not clear whether BCG induces the release of a unique set of cytokines apart from its pro-inflammatory effects. Therefore, we quantified bladder inflammatory responses and alterations in urinary cytokine protein induced by intravesical BCG and compared the results to non-specific pro-inflammatory stimuli (LPS and TNF-alpha). We went further to determine whether BCG treatment alters cytokine gene expression in the urinary bladder.

Methods

C57BL/6 female mice received four weekly instillations of BCG, LPS, or TNF-alpha. Morphometric analys

SUBMITTER: Saban MR 

PROVIDER: S-EPMC1891101 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature