Inducible XIST-dependent X-chromosome inactivation in human somatic cells is reversible.

ABSTRACT: During embryogenesis, the XIST RNA is expressed from and localizes to one X chromosome in females and induces chromosome-wide silencing. Although many changes to inactive X heterochromatin are known, the functional relationships between different modifications are not well understood, and studies of the initiation of X-inactivation have been largely confined to mouse. We now present a model system for human XIST RNA function in which induction of an XIST cDNA in somatic cells results in localized XIST RNA and transcriptional silencing. Chromatin immunoprecipitation and immunohistochemistry shows that this silencing need only be accompanied by a subset of heterochromatic marks and that these can differ between integration sites. Surprisingly, silencing is XIST-dependent, remaining reversibl

SUBMITTER: Chow JC 

PROVIDER: S-EPMC1891207 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature