Rapid bulk endocytosis and its kinetics of fission pore closure at a central synapse.

ABSTRACT: Upon exocytosis, fused vesicles must be retrieved for recycling. One route of retrieval is to generate endosome-like structures, from which small vesicles bud off. Endosome-like structures are widely thought to be generated slowly ( approximately 1 min) from the plasma membrane, a process called bulk endocytosis. Although the concept of bulk endocytosis seems established, the kinetic evidence showing the instant of the bulk membrane fission at synapses is still missing. The present work provides this missing piece of evidence at a calyx-type synapse. We used the capacitance measurement technique, which offers a high time resolution ( approximately 1 ms) to resolve the fission process. The instant of bulk membrane fission was reflected as a brief downward capacitance shift (DCS) of approxim

SUBMITTER: Wu W 

PROVIDER: S-EPMC1891249 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature