NuID: a universal naming scheme of oligonucleotides for illumina, affymetrix, and other microarrays.

ABSTRACT:

Background

Oligonucleotide probes that are sequence identical may have different identifiers between manufacturers and even between different versions of the same company's microarray; and sometimes the same identifier is reused and represents a completely different oligonucleotide, resulting in ambiguity and potentially mis-identification of the genes hybridizing to that probe.

Results

We have devised a unique, non-degenerate encoding scheme that can be used as a universal representation to identify an oligonucleotide across manufacturers. We have named the encoded representation 'nuID', for nucleotide universal identifier. Inspired by the fact that the raw sequence of the oligonucleotide is the true definition of identity for a probe, the encoding algorithm uniquely and no

SUBMITTER: Du P 

PROVIDER: S-EPMC1891274 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature