External guide sequences targeting the aac(6')-Ib mRNA induce inhibition of amikacin resistance.

ABSTRACT: The dissemination of AAC(6')-I-type acetyltransferases have rendered amikacin and other aminoglycosides all but useless in some parts of the world. Antisense technologies could be an alternative to extend the life of these antibiotics. External guide sequences are short antisense oligoribonucleotides that induce RNase P-mediated cleavage of a target RNA by forming a precursor tRNA-like complex. Thirteen-nucleotide external guide sequences complementary to locations within five regions accessible for interaction with antisense oligonucleotides in the mRNA that encodes AAC(6')-Ib were analyzed. While small variations in the location targeted by different external guide sequences resulted in big changes in efficiency of binding to native aac(6')-Ib mRNA, most of them induced high levels of RN

SUBMITTER: Soler Bistue AJ 

PROVIDER: S-EPMC1891410 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature