Expression profiling of ductal carcinoma in situ by laser capture microdissection and high-density oligonucleotide arrays.

ABSTRACT: Gene expression profiling through the use of nucleic acid arrays is a powerful method for the molecular classification of human neoplasms. Laser capture microdissection is an equally useful technique to selectively isolate defined cell populations from heterogeneous histological tissue sections. In this report, we demonstrate how a modest use of laser capture microdissection is sufficient to isolate nanogram quantities of high-quality RNA. Together with the use of several internal standards and microcapillary electrophoresis of input RNA, two rounds of linear molecular amplification have been used to generate sufficient quantities of labeled target for hybridization to high-density oligonucleotide expression arrays. Results demonstrate that the technique is reproducible, generates only mod

SUBMITTER: Luzzi V 

PROVIDER: S-EPMC1891975 | biostudies-literature | 2001 Jun

REPOSITORIES: biostudies-literature