A population study of the minicircles in Trypanosoma cruzi: predicting guide RNAs in the absence of empirical RNA editing.

ABSTRACT:

Background

The structurally complex network of minicircles and maxicircles comprising the mitochondrial DNA of kinetoplastids mirrors the complexity of the RNA editing process that is required for faithful expression of encrypted maxicircle genes. Although a few of the guide RNAs that direct this editing process have been discovered on maxicircles, guide RNAs are mostly found on the minicircles. The nuclear and maxicircle genomes have been sequenced and assembled for Trypanosoma cruzi, the causative agent of Chagas disease, however the complement of 1.4-kb minicircles, carrying four guide RNA genes per molecule in this parasite, has been less thoroughly characterised.

Results

Fifty-four CL Brener and 53 Esmeraldo strain minicircle sequence reads were extracted from T. cruzi

SUBMITTER: Thomas S 

PROVIDER: S-EPMC1892023 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature