No influence of Indy on lifespan in Drosophila after correction for genetic and cytoplasmic background effects.

ABSTRACT: To investigate whether alterations in mitochondrial metabolism affect longevity in Drosophila melanogaster, we studied lifespan in various single gene mutants, using inbred and outbred genetic backgrounds. As positive controls we included the two most intensively studied mutants of Indy, which encodes a Drosophila Krebs cycle intermediate transporter. It has been reported that flies heterozygous for these Indy mutations, which lie outside the coding region, show almost a doubling of lifespan. We report that only one of the two mutants lowers mRNA levels, implying that the lifespan extension observed is not attributable to the Indy mutations themselves. Moreover, neither Indy mutation extended lifespan in female flies in any genetic background tested. In the original genetic background, onl

SUBMITTER: Toivonen JM 

PROVIDER: S-EPMC1892600 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature