A set of stage-specific gene transcripts identified in EK stage X and HH stage 3 chick embryos.

ABSTRACT:

Background

The embryonic developmental process in avian species is quite different from that in mammals. The first cleavage begins 4 h after fertilization, but the first differentiation does not occur until laying of the egg (Eyal-Giladi and Kochav (EK) stage X). After 12 to 13 h of incubation (Hamburger and Hamilton (HH) stage 3), the three germ layers form and germ cell segregation in the early chick embryo are completed. Thus, to identify genes associated with early embryonic development, we compared transcript expression patterns between undifferentiated (stage X) and differentiated (HH stage 3) embryos.

Results

Microarray analysis primarily showed 40 genes indicating the significant changes in expression levels between stage X and HH stage 3, and 80% of the genes (32/40

SUBMITTER: Lee BR 

PROVIDER: S-EPMC1894632 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature