Global repeat discovery and estimation of genomic copy number in a large, complex genome using a high-throughput 454 sequence survey.

ABSTRACT:

Background

Extensive computational and database tools are available to mine genomic and genetic databases for model organisms, but little genomic data is available for many species of ecological or agricultural significance, especially those with large genomes. Genome surveys using conventional sequencing techniques are powerful, particularly for detecting sequences present in many copies per genome. However these methods are time-consuming and have potential drawbacks. High throughput 454 sequencing provides an alternative method by which much information can be gained quickly and cheaply from high-coverage surveys of genomic DNA.

Results

We sequenced 78 million base-pairs of randomly sheared soybean DNA which passed our quality criteria. Computational analysis of the surve

SUBMITTER: Swaminathan K 

PROVIDER: S-EPMC1894642 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature