A full-genomic sequence-verified protein-coding gene collection for Francisella tularensis.

ABSTRACT: The rapid development of new technologies for the high throughput (HT) study of proteins has increased the demand for comprehensive plasmid clone resources that support protein expression. These clones must be full-length, sequence-verified and in a flexible format. The generation of these resources requires automated pipelines supported by software management systems. Although the availability of clone resources is growing, current collections are either not complete or not fully sequence-verified. We report an automated pipeline, supported by several software applications that enabled the construction of the first comprehensive sequence-verified plasmid clone resource for more than 96% of protein coding sequences of the genome of F. tularensis, a highly virulent human pathogen and the ca

SUBMITTER: Murthy T 

PROVIDER: S-EPMC1894649 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature