Recruitment of Polo kinase to the spindle midzone during cytokinesis requires the Feo/Klp3A complex.

ABSTRACT:

Background

Polo-like kinases control multiple events during cell division, including mitotic entry, centrosome organization, spindle formation, chromosome segregation and cytokinesis. Their roles during cytokinesis, however, are not well understood because the requirement of these kinases during early stages of mitosis complicates the study of their functions after anaphase onset.

Methodology/principal findings

We used time-lapse microscopy to analyze the dynamics of Polo::GFP in Drosophila tissue culture cells during mitosis. After anaphase onset, Polo::GFP concentrated at the spindle midzone, but also diffused along the entire length of the central spindle. Using RNA interference we demonstrate that the microtubule-associated proteins Feo and Klp3A are required for Polo re

SUBMITTER: D'Avino PP 

PROVIDER: S-EPMC1894651 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature