NGF-mediated transcriptional targets of p53 in PC12 neuronal differentiation.

ABSTRACT:

Background

p53 is recognized as a critical regulator of the cell cycle and apoptosis. Mounting evidence also suggests a role for p53 in differentiation of cells including neuronal precursors. We studied the transcriptional role of p53 during nerve growth factor-induced differentiation of the PC12 line into neuron-like cells. We hypothesized that p53 contributed to PC12 differentiation through the regulation of gene targets distinct from its known transcriptional targets for apoptosis or DNA repair.

Results

Using a genome-wide chromatin immunoprecipitation cloning technique, we identified and validated 14 novel p53-regulated genes following NGF treatment. The data show p53 protein was transcriptionally activated and contributed to NGF-mediated neurite outgrowth during differe

SUBMITTER: Brynczka C 

PROVIDER: S-EPMC1894799 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature