Production of surfactin from Bacillus subtilis MZ-7 grown on pharmamedia commercial medium.

ABSTRACT:

Background

Commercial medium (Pharmamedia) was investigated for the production of surfactin by Bacillus subtilis MZ-7. Different media (defined, semi-defined, and complex media) were compared for the production of surfactin after fixing the least influential variables in standardized fermentation conditions. Carbohydrate and nitrogen supplements were also tried to improve production in Pharmamedia.

Results

Surfactin production was confirmed using PCR along with other analytical techniques and monitored by RP-HPLC and MALDI-TOF-MS. We found that optimized and brain heart infusion media were best for production of surfactin (280 mg/L) and a relatively comparable production with Pharmamedia (220 mg/L), however, supplementing Pharmamedia with Fe+ (4.0 mM) and sucrose (2 g/L) lea

SUBMITTER: Al-Ajlani MM 

PROVIDER: S-EPMC1894814 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature