Gene expression profiles of Chlamydophila pneumoniae during the developmental cycle and iron depletion-mediated persistence.

ABSTRACT: The obligate intracellular, gram-negative bacterium Chlamydophila pneumoniae (Cpn) has impact as a human pathogen. Little is known about changes in the Cpn transcriptome during its biphasic developmental cycle (the acute infection) and persistence. The latter stage has been linked to chronic diseases. To analyze Cpn CWL029 gene expression, we designed a pathogen-specific oligo microarray and optimized the extraction method for pathogen RNA. Throughout the acute infection, ratio expression profiles for each gene were generated using 48 h post infection as a reference. Based on these profiles, significantly expressed genes were separated into 12 expression clusters using self-organizing map clustering and manual sorting into the "early", "mid", "late", and "tardy" cluster classes. The latter

SUBMITTER: Maurer AP 

PROVIDER: S-EPMC1894823 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature