Genomic analysis of the secretion stress response in the enzyme-producing cell factory Aspergillus niger.

ABSTRACT:

Background

Filamentous fungi such as Aspergillus niger have a high capacity secretory system and are therefore widely exploited for the industrial production of native and heterologous proteins. However, in most cases the yields of non-fungal proteins are significantly lower than those obtained for fungal proteins. One well-studied bottleneck appears to be the result of mis-folding of heterologous proteins in the ER during early stages of secretion, with related stress responses in the host, including the unfolded protein response (UPR). This study aims at uncovering transcriptional and translational responses occurring in A. niger exposed to secretion stress.

Results

A genome-wide transcriptional analysis of protein secretion-related stress responses was determined using Af

SUBMITTER: Guillemette T 

PROVIDER: S-EPMC1894978 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature