Arsenic suppresses gene expression in promyelocytic leukemia cells partly through Sp1 oxidation.

ABSTRACT: The mechanism by which arsenic dramatically affects gene expression remains poorly understood. Here we report that prolonged exposure of acute promyelocytic leukemia NB4 cells to low levels of arsenic trioxide increased the expression of a set of genes responsible for reactive oxygen species (ROS) production. We hypothesize that arsenic-induced ROS in turn contribute partially to altered gene expression. To identify genes responsive to arsenic-induced ROS, we used microarray gene expression analysis and identified genes that responded to arsenic and hydrogen peroxide but whose response to arsenic was reversed by an ROS scavenger, N-acetyl-L-cysteine. We found that 26% of the genes significantly responsive to arsenic might have been directly altered by ROS. We further explored the mechanism

SUBMITTER: Chou WC 

PROVIDER: S-EPMC1895131 | biostudies-literature | 2005 Jul

REPOSITORIES: biostudies-literature