Cooperating cancer-gene identification through oncogenic-retrovirus-induced insertional mutagenesis.

ABSTRACT: Multiple cooperating mutations that deregulate different signaling pathways are required to induce cancer. Identifying these cooperating mutations is a prerequisite for developing better combinatorial therapies for treating cancer. Here we show that cooperating cancer mutations can be identified through oncogenic-retrovirus-induced insertional mutagenesis. Among 13 myeloid leukemias induced by transplanting into mice bone marrow cells infected in vitro with a replication-defective retrovirus carrying the Sox4 oncogene, 9 contained insertional mutations at known or suspected cancer genes. This likely occurred because rare bone marrow cells, in which the oncogenic retrovirus happened to integrate and in which it mutated a cooperating cancer gene, were selected because the host harbored a coo

SUBMITTER: Du Y 

PROVIDER: S-EPMC1895273 | biostudies-literature | 2005 Oct

REPOSITORIES: biostudies-literature