Protein-tyrosine kinase activity profiling in knock down zebrafish embryos.

ABSTRACT:

Background

Protein-tyrosine kinases (PTKs) regulate virtually all biological processes. PTKs phosphorylate substrates in a sequence-specific manner and relatively short peptide sequences determine selectivity. Here, we developed new technology to determine PTK activity profiles using peptide arrays. The zebrafish is an excellent model system to investigate signaling in the whole organism, given its wealth of genetic tools, including morpholino-mediated knock down technology. We used zebrafish embryo lysates to determine PTK activity profiles, thus providing the unique opportunity to directly compare the effect of protein knock downs on PTK activity profiles on the one hand and phenotypic changes on the other.

Methodology

We used multiplex arrays of 144 distinct peptides, spo

SUBMITTER: Lemeer S 

PROVIDER: S-EPMC1895888 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature