Array feature size influences nucleic acid surface capture in DNA microarrays.

ABSTRACT: Analyte affinity capture by surface-immobilized diagnostic agents is a routinely used assay format for profiling numerous medically and technologically important target analytes. These assays suffer from numerous performance limitations, including sensitivity and rapidity. Assay miniaturization is advocated to improve surface-capture performance, specifically exploiting the inverse relationship between analyte flux and capture feature size under mass transfer-limiting capture conditions that characterize many such assay formats. Reduced capture feature sizes, e.g., microarrays, are proposed to overcome mass transfer limitations, yet this is difficult to achieve across several size scales. This study validates certain advantages advocated for capture spot miniaturization using a rationale t

SUBMITTER: Dandy DS 

PROVIDER: S-EPMC1895933 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature