Discovery of tissue-specific exons using comprehensive human exon microarrays.

ABSTRACT:

Background

Higher eukaryotes express a diverse population of messenger RNAs generated by alternative splicing. Large-scale methods for monitoring gene expression must adapt in order to accurately detect the transcript variation generated by this splicing.

Results

We have designed a high-density oligonucleotide microarray with probesets for more than one million annotated and predicted exons in the human genome. Using these arrays and a simple algorithm that normalizes exon signal to signal from the gene as a whole, we have identified tissue-specific exons from a panel of 16 different normal adult tissues. RT-PCR validation confirms approximately 86% of the predicted tissue-enriched probesets. Pair-wise comparisons between the tissues suggest that as many as 73% of detected g

SUBMITTER: Clark TA 

PROVIDER: S-EPMC1896007 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature