Global analyses of mRNA translational control during early Drosophila embryogenesis.

ABSTRACT:

Background

In many animals, the first few hours of life proceed with little or no transcription, and developmental regulation at these early stages is dependent on maternal cytoplasm rather than the zygotic nucleus. Translational control is critical for early Drosophila embryogenesis and is exerted mainly at the gene level. To understand post-transcriptional regulation during Drosophila early embryonic development, we used sucrose polysomal gradient analyses and GeneChip analysis to illustrate the translation profile of individual mRNAs.

Results

We determined ribosomal density and ribosomal occupancy of over 10,000 transcripts during the first ten hours after egg laying.

Conclusion

We report the extent and general nature of gene regulation at the translational level d

SUBMITTER: Qin X 

PROVIDER: S-EPMC1896012 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature