Validation of reference genes for quantitative RT-PCR studies in porcine oocytes and preimplantation embryos.

ABSTRACT:

Background

In the developing embryo, total RNA abundance fluctuates caused by functional RNA degradation and zygotic genome activation. These variations in the transcriptome in early development complicate the choice of good reference genes for gene expression studies by quantitative real time polymerase chain reaction.

Results

In order to identify stably expressed genes for normalisation of quantitative data, within early stages of development, transcription levels were examined of 7 frequently used reference genes (B2M, BACT, GAPDH, H2A, PGK1, SI8, and UBC) at different stages of early porcine embryonic development (germinal vesicle, metaphase-2, 2-cell, 4-cell, early blastocyst, expanded blastocyst). Analysis of transcription profiling by geNorm software revealed that GAP

SUBMITTER: Kuijk EW 

PROVIDER: S-EPMC1896162 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature