SIR: siRNA Information Resource, a web-based tool for siRNA sequence design and analysis and an open access siRNA database.

ABSTRACT:

Background

RNA interference has revolutionized our ability to study the effects of altering the expression of single genes in mammalian (and other) cells through targeted knockdown of gene expression. In this report we describe a web-based computational tool, siRNA Information Resource (sIR), which consists of a new open source database that contains validation information about published siRNA sequences and also provides a user-friendly interface to design and analyze siRNA sequences against a chosen target sequence.

Results

The siRNA design tool described in this paper employs empirically determined rules derived from a meta-analysis of the published data; it uses a weighted scoring system that determines the optimal sequence within a target mRNA and thus aids in the ratio

SUBMITTER: Shah JK 

PROVIDER: S-EPMC1896181 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature