The chemokine receptor D6 has opposing effects on allergic inflammation and airway reactivity.

ABSTRACT:

Rationale

The D6 chemokine receptor can bind and scavenge several chemokines, including the T-helper 2 (Th2)-associated chemokines CCL17 and CCL22. Although D6 is constitutively expressed in the lung, its pulmonary function is unknown.

Objectives

This study tested whether D6 regulates pulmonary chemokine levels, inflammation, or airway responsiveness during allergen-induced airway disease.

Methods

D6-deficient and genetically matched C57BL/6 mice were sensitized and challenged with ovalbumin. ELISA and flow cytometry were used to measure levels of cytokines and leukocytes, respectively. Mechanical ventilation was used to measure airway reactivity.

Results

The ability of D6 to diminish chemokine levels in the lung was chemokine concentration dependent. CCL17 and

SUBMITTER: Whitehead GS 

PROVIDER: S-EPMC1899265 | biostudies-literature | 2007 Feb

REPOSITORIES: biostudies-literature