Impact of low and high tidal volumes on the rat alveolar epithelial type II cell proteome.

ABSTRACT:

Rationale

Mechanical ventilation with high tidal volumes leads to increased permeability, generation of inflammatory mediators, and damage to alveolar epithelial cells (ATII).

Objectives

To identify changes in the ATII proteome after two different ventilation strategies in rats.

Methods

Rats (n = 6) were ventilated for 5 hours with high- and low tidal volumes (VTs) (high VT: 20 ml/kg; low VT: 6 ml/kg). Pooled nonventilated rats served as control animals. ATII cells were isolated and lysed, and proteins were tryptically cleaved into peptides. Cellular protein content was evaluated by peptide labeling of the ventilated groups with (18)O. Samples were fractionated by cation exchange chromatography and identified using electrospray tandem mass spectrometry. Proteins ident

SUBMITTER: Hirsch J 

PROVIDER: S-EPMC1899270 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature