Nuclear factor-kappaB activation in neonatal mouse lung protects against lipopolysaccharide-induced inflammation.

ABSTRACT:

Rationale

Injurious agents often cause less severe injury in neonates as compared with adults.

Objective

We hypothesized that maturational differences in lung inflammation induced by lipopolysaccharide (LPS) may be related to the nature of the nuclear factor (NF)-kappaB complex activated, and the profile of target genes expressed.

Methods

Neonatal and adult mice were injected with intraperitoneal LPS. Lung inflammation was assessed by histology, and apoptosis was determined by TUNEL (terminal deoxynucleotidyl transferase UTP nick-end labeling). The expression of candidate inflammatory and apoptotic mediators was evaluated by quantitative real-time polymerase chain reaction and Western immunoblot.

Results

Neonates demonstrated reduced inflammation and apoptosis,

SUBMITTER: Alvira CM 

PROVIDER: S-EPMC1899293 | biostudies-literature | 2007 Apr

REPOSITORIES: biostudies-literature