Allele-specific binding of airway nuclear extracts to polymorphic beta2-adrenergic receptor 5' sequence.

ABSTRACT: Like other intronless G protein-coupled receptor genes, the beta(2)-adrenergic receptor (beta(2)AR) has minimal genetic space for population variability, and has attained such via multiple coding and noncoding polymorphisms. Yet most clinical studies use the two nonsynonymous polymorphisms of the coding region for association analysis despite low levels of linkage disequilibrium with some promoter and 5'UTR polymorphisms. To assess the potential for allele-specific transcription factor binding to beta(2)AR 5'-flanking sequence, 3'-biotin-labeled oligonucleotide duplexes were synthesized. Each was centered on variable sites representing major or minor alleles found in the human population with frequencies of 5% or greater (20 polymorphic sites). Electrophoretic mobility shift assays were pe

SUBMITTER: Panebra A 

PROVIDER: S-EPMC1899344 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature