The role of platelets in leukocyte recruitment in chronic contact hypersensitivity induced by repeated elicitation.

ABSTRACT: Platelets have been shown to be important in inflammation, but their role in chronic allergic dermatitis remains unclear. To investigate the role of platelets in a mouse model of chronic contact hypersensitivity induced by repeated elicitation, mice were sensitized and repeatedly elicited in ears with hapten, with or without platelet depletion, by administering antiplatelet antibody or busulfan. Ear thickness, leukocyte infiltration, serum IgE, and scratching behavior significantly decreased in thrombocytopenic mice. cDNA microarray of ear tissue showed reduced gene expression associated with Th2 lymphocytes. Flow cytometry showed increased P-selectin expression on platelets and an increased number of platelet-leukocyte aggregates in blood of repeatedly elicited mice, compared with sham-se

SUBMITTER: Tamagawa-Mineoka R 

PROVIDER: S-EPMC1899463 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature