Identification and expression analysis of genes associated with bovine blastocyst formation.

ABSTRACT:

Background

Normal preimplantation embryo development encompasses a series of events including first cleavage division, activation of the embryonic genome, compaction and blastocyst formation. First lineage differentiation starts at the blastocyst stage with the formation of the trophectoderm and the inner cell mass. The main objective of this study was the detection, identification and expression analysis of genes associated with blastocyst formation in order to help us better understand this process. This information could lead to improvements of in vitro embryo production procedures.

Results

A subtractive cDNA library was constructed enriched for transcripts preferentially expressed at the blastocyst stage compared to the 2-cell and 8-cell stage. Sequence information was o

SUBMITTER: Goossens K 

PROVIDER: S-EPMC1899496 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature