Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.

ABSTRACT: Faithful partitioning of genetic material during cell division requires accurate spatial and temporal positioning of nuclei within dividing cells. In Saccharomyces cerevisiae, nuclear positioning is regulated by an elegant interplay between components of the actin and microtubule cytoskeletons. Regulators of this process include Bud6p (also referred to as the actin-interacting protein Aip3p) and Kar9p, which function to promote contacts between cytoplasmic microtubule ends and actin-delimited cortical attachment points. Here, we present the previously undetected association of Bud6p with the cytoplasmic face of yeast spindle pole bodies, the functional equivalent of metazoan centrosomes. Cells lacking Bud6p show exaggerated movements of the nucleus between mother and daughter cells and dis

SUBMITTER: Haarer BK 

PROVIDER: S-EPMC1899834 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature