Secretion of the adipocyte-specific secretory protein adiponectin critically depends on thiol-mediated protein retention.

ABSTRACT: Adiponectin is a secretory protein abundantly secreted from adipocytes. It assembles into a number of different higher-order complexes. Adipocytes maintain tight control over circulating plasma levels, suggesting the existence of a complex, highly regulated biosynthetic pathway. However, the critical mediators of adiponectin maturation within the secretory pathway have not been elucidated. Previously, we found that a significant portion of de novo-synthesized adiponectin is not secreted and retained in adipocytes. Here, we show that there is an abundant pool of properly folded adiponectin in the secretory pathway that is retained through thiol-mediated retention, as judged by the release of adiponectin in response to treatment of adipocytes with reducing agents. Adiponectin is covalently b

SUBMITTER: Wang ZV 

PROVIDER: S-EPMC1899995 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature