The evolutionary conservation of the core components necessary for the extrinsic apoptotic signaling pathway, in Medaka fish.

ABSTRACT:

Background

Death receptors on the cell surface and the interacting cytosolic molecules, adaptors and initiator caspases, are essential as core components of the extrinsic apoptotic signaling pathway. While the apoptotic machinery governing the extrinsic signaling pathway is well characterized in mammals, it is not fully understood in fish.

Results

We identified and characterized orthologs of mammalian Fas, FADD and caspase-8 that correspond to the death receptor, adaptor and initiator caspase, from the Medaka fish (Oryzias latipes). Medaka Fas, caspase-8 and FADD exhibited protein structures similar to that of their mammalian counterparts, containing a death domain (DD), a death effector domain (DED) or both. Functional analyses indicated that these molecules possess killing

SUBMITTER: Sakamaki K 

PROVIDER: S-EPMC1903365 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature