Transposition of the rice miniature inverted repeat transposable element mPing in Arabidopsis thaliana.

ABSTRACT: An active miniature inverted repeat transposable element (MITE), mPing, was discovered by computer-assisted analysis of rice genome sequence. The mPing element is mobile in rice cell culture and in a few rice strains where it has been amplified to >1,000 copies during recent domestication. However, determination of the transposase source and characterization of the mechanism of transposition have been hampered by the high copy number of mPing and the presence of several candidate autonomous elements in the rice genome. Here, we report that mPing is active in Arabidopsis thaliana, where its transposition is catalyzed by three sources of transposase from rice: the autonomous Ping and Pong elements and by a cDNA derived from a Ping transcript. In addition to transposase, the product of a seco

SUBMITTER: Yang G 

PROVIDER: S-EPMC1904124 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature