The red fluorescent protein eqFP611: application in subcellular localization studies in higher plants.

ABSTRACT:

Background

Intrinsically fluorescent proteins have revolutionized studies in molecular cell biology. The parallel application of these proteins in dual- or multilabeling experiments such as subcellular localization studies requires non-overlapping emission spectra for unambiguous detection of each label. In the red spectral range, almost exclusively DsRed and derivatives thereof are used today. To test the suitability of the red fluorescent protein eqFP611 as an alternative in higher plants, the behavior of this protein was analyzed in terms of expression, subcellular targeting and compatibility with GFP in tobacco.

Results

When expressed transiently in tobacco protoplasts, eqFP611 accumulated over night to levels easily detectable by fluorescence microscopy. The native prot

SUBMITTER: Forner J 

PROVIDER: S-EPMC1904219 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature