Dynamic resolution of functionally related gene sets in response to acute heat stress.

ABSTRACT:

Background

Using a gene clustering strategy we determined intracellular pathway relationships within skeletal myotubes in response to an acute heat stress stimuli. Following heat shock, the transcriptome was analyzed by microarray in a temporal fashion to characterize the dynamic relationship of signaling pathways.

Results

Bioinformatics analyses exposed coordination of functionally-related gene sets, depicting mechanism-based responses to heat shock. Protein turnover-related pathways were significantly affected including protein folding, pre-mRNA processing, mRNA splicing, proteolysis and proteasome-related pathways. Many responses were transient, tending to normalize within 24 hours.

Conclusion

In summary, we show that the transcriptional response to acute cell stre

SUBMITTER: Szustakowski JD 

PROVIDER: S-EPMC1904231 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature