Transcription-coupled deposition of histone modifications during MHC class II gene activation.

ABSTRACT: Posttranslational histone modifications associated with actively expressed genes are generally believed to be introduced primarily by histone-modifying enzymes that are recruited by transcription factors or their associated co-activators. We have performed a comprehensive spatial and temporal analyses of the histone modifications that are deposited upon activation of the MHC class II gene HLA-DRA by the co-activator CIITA. We find that transcription-associated histone modifications are introduced during two sequential phases. The first phase precedes transcription initiation and is characterized exclusively by a rapid increase in histone H4 acetylation over a large upstream domain. All other modifications examined, including the acetylation and methylation of several residues in histone H3

SUBMITTER: Rybtsova N 

PROVIDER: S-EPMC1904273 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature