Lateral flow microarrays: a novel platform for rapid nucleic acid detection based on miniaturized lateral flow chromatography.

ABSTRACT: Widely used nucleic acid assays are poorly suited for field deployment where access to laboratory instrumentation is limited or unavailable. The need for field deployable nucleic acid detection demands inexpensive, facile systems without sacrificing information capacity or sensitivity. Here we describe a novel microarray platform capable of rapid, sensitive nucleic acid detection without specialized instrumentation. The approach is based on a miniaturized lateral flow device that makes use of hybridization-mediated target capture. The miniaturization of lateral flow nucleic acid detection provides multiple advantages over traditional lateral flow devices. Ten-microliter sample volumes reduce reagent consumption and yield analyte detection times, excluding sample preparation and amplificati

SUBMITTER: Carter DJ 

PROVIDER: S-EPMC1904290 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature