Laser microdissection of narrow sheath mutant maize uncovers novel gene expression in the shoot apical meristem.

ABSTRACT: Microarrays enable comparative analyses of gene expression on a genomic scale, however these experiments frequently identify an abundance of differentially expressed genes such that it may be difficult to identify discrete functional networks that are hidden within large microarray datasets. Microarray analyses in which mutant organisms are compared to nonmutant siblings can be especially problematic when the gene of interest is expressed in relatively few cells. Here, we describe the use of laser microdissection microarray to perform transcriptional profiling of the maize shoot apical meristem (SAM), a ~100-microm pillar of organogenic cells that is required for leaf initiation. Microarray analyses compared differential gene expression within the SAM and incipient leaf primordium of nonmu

SUBMITTER: Zhang X 

PROVIDER: S-EPMC1904365 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature