Genome-wide fitness test and mechanism-of-action studies of inhibitory compounds in Candida albicans.

ABSTRACT: Candida albicans is a prevalent fungal pathogen amongst the immunocompromised population, causing both superficial and life-threatening infections. Since C. albicans is diploid, classical transmission genetics can not be performed to study specific aspects of its biology and pathogenesis. Here, we exploit the diploid status of C. albicans by constructing a library of 2,868 heterozygous deletion mutants and screening this collection using 35 known or novel compounds to survey chemically induced haploinsufficiency in the pathogen. In this reverse genetic assay termed the fitness test, genes related to the mechanism of action of the probe compounds are clearly identified, supporting their functional roles and genetic interactions. In this report, chemical-genetic relationships are provided fo

SUBMITTER: Xu D 

PROVIDER: S-EPMC1904411 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature