Rapid detection of Mycobacterium bovis DNA in cattle lymph nodes with visible lesions using PCR.

ABSTRACT:

Background

We have evaluated a sensitive screening assay for Mycobacterium tuberculosis (MTB) complex organisms and a specific assay for detecting Mycobacterium bovis DNA in lymph nodes taken from cattle with evidence of bovine tuberculosis. Underlying these series of experiments was the need for a versatile DNA extraction protocol which could handle tissue samples and with the potential for automation.The target for the screening assay was the multi-copy insertion element IS1081, present in 6 copies in the MTB complex. For confirmation of M. bovis we used primers flanking a specific deletion in the genome of M. bovis known as region of difference 4 (RD4). The sensitivity and specificity of these PCRs has been tested on genomic DNA from MTB complex reference strains, mycobacteria o

SUBMITTER: Taylor GM 

PROVIDER: S-EPMC1904440 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature