GOGOT: a method for the identification of differentially expressed fragments from cDNA-AFLP data.

ABSTRACT:

Background

One-dimensional (1-D) electrophoretic data obtained using the cDNA-AFLP method have attracted great interest for the identification of differentially expressed transcript-derived fragments (TDFs). However, high-throughput analysis of the cDNA-AFLP data is currently limited by the need for labor-intensive visual evaluation of multiple electropherograms. We would like to have high-throughput ways of identifying such TDFs.

Results

We describe a method, GOGOT, which automatically detects the differentially expressed TDFs in a set of time-course electropherograms. Analysis by GOGOT is conducted as follows: correction of fragment lengths of TDFs, alignment of identical TDFs across different electropherograms, normalization of peak heights, and identification of differen

SUBMITTER: Kadota K 

PROVIDER: S-EPMC1904450 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature