Generation and analysis of expressed sequence tags from the ciliate protozoan parasite Ichthyophthirius multifiliis.

ABSTRACT:

Background

The ciliate protozoan Ichthyophthirius multifiliis (Ich) is an important parasite of freshwater fish that causes 'white spot disease' leading to significant losses. A genomic resource for large-scale studies of this parasite has been lacking. To study gene expression involved in Ich pathogenesis and virulence, our goal was to generate expressed sequence tags (ESTs) for the development of a powerful microarray platform for the analysis of global gene expression in this species. Here, we initiated a project to sequence and analyze over 10,000 ESTs.

Results

We sequenced 10,368 EST clones using a normalized cDNA library made from pooled samples of the trophont, tomont, and theront life-cycle stages, and generated 9,769 sequences (94.2% success rate). Post-sequencing p

SUBMITTER: Abernathy JW 

PROVIDER: S-EPMC1906770 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature