Towards the ictalurid catfish transcriptome: generation and analysis of 31,215 catfish ESTs.

ABSTRACT:

Background

EST sequencing is one of the most efficient means for gene discovery and molecular marker development, and can be additionally utilized in both comparative genome analysis and evaluation of gene duplications. While much progress has been made in catfish genomics, large-scale EST resources have been lacking. The objectives of this project were to construct primary cDNA libraries, to conduct initial EST sequencing to generate catfish EST resources, and to obtain baseline information about highly expressed genes in various catfish organs to provide a guide for the production of normalized and subtracted cDNA libraries for large-scale transcriptome analysis in catfish.

Results

A total of 17 cDNA libraries were constructed including 12 from channel catfish (Ictalurus p

SUBMITTER: Li P 

PROVIDER: S-EPMC1906771 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature