Modeling insertional mutagenesis using gene length and expression in murine embryonic stem cells.

ABSTRACT:

Background

High-throughput mutagenesis of the mammalian genome is a powerful means to facilitate analysis of gene function. Gene trapping in embryonic stem cells (ESCs) is the most widely used form of insertional mutagenesis in mammals. However, the rules governing its efficiency are not fully understood, and the effects of vector design on the likelihood of gene-trapping events have not been tested on a genome-wide scale.

Methodology/principal findings

In this study, we used public gene-trap data to model gene-trap likelihood. Using the association of gene length and gene expression with gene-trap likelihood, we constructed spline-based regression models that characterize which genes are susceptible and which genes are resistant to gene-trapping techniques. We report result

SUBMITTER: Nord AS 

PROVIDER: S-EPMC1910612 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature