Translational control of recombinant human acetylcholinesterase accumulation in plants.

ABSTRACT:

Background

Codon usage differences are known to regulate the levels of gene expression in a species-specific manner, with the primary factors often cited to be mRNA processing and accumulation. We have challenged this conclusion by expressing the human acetylcholinesterase coding sequence in transgenic plants in its native GC-rich sequence and compared to a matched sequence with (dicotyledonous) plant-optimized codon usage and a lower GC content.

Results

We demonstrate a 5 to 10 fold increase in accumulation levels of the "synaptic" splice variant of human acetylcholinesterase in Nicotiana benthamiana plants expressing the optimized gene as compared to the native human sequence. Both transient expression assays and stable transformants demonstrated conspicuously increased ac

SUBMITTER: Geyer BC 

PROVIDER: S-EPMC1913049 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature