Genetic analysis of Helicobacter pylori strain populations colonizing the stomach at different times postinfection.

ABSTRACT: Genetic diversity of the human gastric pathogen Helicobacter pylori in an individual host has been observed; whether this diversity represents diversification of a founding strain or a mixed infection with distinct strain populations is not clear. To examine this issue, we analyzed multiple single-colony isolates from two to four separate stomach biopsies of eight adult and four pediatric patients from a high-incidence Mexican population. Eleven of the 12 patients contained isolates with identical random amplified polymorphic DNA, amplified fragment length polymorphism, and vacA allele molecular footprints, whereas a single adult patient had two distinct profiles. Comparative genomic hybridization using whole-genome microarrays (array CGH) revealed variation in 24 to 67 genes in isolates f

SUBMITTER: Salama NR 

PROVIDER: S-EPMC1913316 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature