Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology.

ABSTRACT: Individual gene-targeted hpn and hpn-like mutants and a mutant with mutations in both hpn genes were more sensitive to nickel, cobalt, and cadmium toxicity than was the parent strain, with the hpn-like strain showing the most metal sensitivity of the two individual His-rich protein mutants. The mutant strains contained up to eightfold more urease activity than the parent under nickel-deficient conditions, and the parent strain was able to achieve mutant strain activity levels by nickel supplementation. The mutants contained 3- to 4-fold more and the double mutant about 10-fold more Ni associated with their total urease pools, even though all of the strains expressed similar levels of total urease protein. Hydrogenase activities in the mutants were like those in the parent strain; thus, hyd

SUBMITTER: Seshadri S 

PROVIDER: S-EPMC1913394 | biostudies-literature | 2007 Jun

REPOSITORIES: biostudies-literature